Applications of Phase Contrast and Fluorescent Microscopy - Virtual Fluorescent Microscope

Applications of Phase Contrast and Fluorescent Microscopy - Virtual Fluorescent Microscope - Wartburg Biology Department

Applications of Phase Contrast
and Fluorescent Microscopy

Filter Cube	Fluorochrome	Use
FITC B	Acridine orange Alexa Fluor 488 Phallodium Bodipy FL Bodipy FL C5 Ceramide SYTO 9 and propidium iodide Fluoroscein SYBR Green Alexa Fluor 488 Anitbody	Stains DNA, count bacteria in natural samples Stains F-actin, used to detect microfilaments in eukaryotic cells Lysosomes Golgi Live/Dead staining of bacteria Immunoflourescence Tag Comet Assay (DNA Damage) Proliferation Assay
TRITC G	Cy 3 Mitotraker Rhodamine	Stains amino groups and used to label RNA probes for FISH (fluorescent in-situ hydridization) methods. Stains mitochondria (live) and then will remain stained after fixation Immunofluorescence tag.
DAPI U		Stains DNA, count bacteria in natural samples Use as a counterstain for nuclei in eukaryotic cells.